Application notes

Written by scientists for scientists, these papers cover a wide range of applications and highlight novel ways to optimize one’s research with our devices, technologies and consumables.

Researchers at BAM successfully developed a novel method combining single cell isolation and arraying by cellenONE® and subsequent isotopic fingerprinting of these single cells by laser ablation ICP-TOF mass spectrometry.

Arraying of single cells for quantitative high throughput Laser Ablation ICP-TOF-MS

Researchers at BAM successfully developed a novel method combining single cell isolation and arraying by cellenONE® and subsequent isotopic fingerprinting of these single cells by laser ablation ICP-TOF mass spectrometry.
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Unlike flow cell sorters, which induce high stress and compromise cell viabilities, automated cloning using cellenONE is a gentle and precise method to achieve unparalleled clonal recoveries in both standard (96 MTP) and high density (1536 MTP) substrates.

cellenONE® Unparalleled clonal recovery achieved in 96 and 1536 Microtiter Plates

Unlike flow cell sorters, which induce high stress and compromise cell viabilities, automated cloning using cellenONE is a gentle and precise method to achieve unparalleled clonal recoveries in both standard (96 MTP) and high density (1536 MTP) substrates.
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Time- and cost-efficient production of high-expressing and fast-expanding monoclonal cell lines using the MACSQuant® Tyto® Cell Sorter in combination with the cellenONE® X1 single-cell deposition unit.

Cell-line Development with Miltenyi TYTO sorting and cellenONE

Time- and cost-efficient production of high-expressing and fast-expanding monoclonal cell lines using the MACSQuant® Tyto® Cell Sorter in combination with the cellenONE® X1 single-cell deposition unit.
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An essential component of today's research in life science is the use of automated liquid handling equipment. The transferred volumes are becoming increasingly small down to nano- and picoliter range.

Performance evaluation for SCIENIONs sciDROP PICO and sciDROP NANO technology using the Artel dual-dye ratiometric photometry

An essential component of today's research in life science is the use of automated liquid handling equipment. The transferred volumes are becoming increasingly small down to nano- and picoliter range.
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In order to use antibodies as diagnostic markers for the prediction of both prognosis and therapeutic response, accurate characterization of their antigen binding region is indispensable. To this end, comprehensive but differentiated immunoassays need to be developed.

Peptide microarrays for epitope mapping of antibodies against hTSHR

In order to use antibodies as diagnostic markers for the prediction of both prognosis and therapeutic response, accurate characterization of their antigen binding region is indispensable. To this end, comprehensive but differentiated immunoassays need to be developed.
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Diagnostic methods can be used to detect all kinds of components such as proteins (biomarkers, toxins, food allergens), microorganisms, specific RNA/DNA sequences, toxic chemicals (plasticizers), contaminants (antibiotics/pesticides), and other quality-determining components.

Rapid and multi-analyte diagnostic microarrays

Diagnostic methods can be used to detect all kinds of components such as proteins (biomarkers, toxins, food allergens), microorganisms, specific RNA/DNA sequences, toxic chemicals (plasticizers), contaminants (antibiotics/pesticides), and other quality-determining components.
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Reliable production of biochips depends on many parameters. In the DNA world, glass supports with different coupling chemistries are well established, whereas Nitrocellulose Membranes (NC) are the favoured support for protein biochips.

Comprehensive comparison of protein microarray supports using contact and non-contact systems

Reliable production of biochips depends on many parameters. In the DNA world, glass supports with different coupling chemistries are well established, whereas Nitrocellulose Membranes (NC) are the favoured support for protein biochips.
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The objective of this project was to develop a new cost-effective Multiplex test to confirm HTLV infection and discriminate the two strains HTLV-1 and HTLV-2.

A novel high performing Multiplex Immunoassay for the Serological Confirmation and Typing of HTLV Infections

The objective of this project was to develop a new cost-effective Multiplex test to confirm HTLV infection and discriminate the two strains HTLV-1 and HTLV-2.
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The prime motivator for transferring traditional ELISA assays to a miniaturized multiplex ELISA format is the significant saving of time, assay materials and patient samples.

Multiplexed ELISA of cardiovascular disease biomarkers in 96 well plate

The prime motivator for transferring traditional ELISA assays to a miniaturized multiplex ELISA format is the significant saving of time, assay materials and patient samples.
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In clinical chemistry, standard ELISA and Western blotting techniques are often used to determine and quantify inflammation markers. However, these techniques can only measure a single parameter per assay and well.

Optimization of array – based diagnostic ELISA tests for the sensitive quantification of three inflammation markers

In clinical chemistry, standard ELISA and Western blotting techniques are often used to determine and quantify inflammation markers. However, these techniques can only measure a single parameter per assay and well.
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To cover applications demanding large volume dispensing, SCIENION recently launched sciDROP NANO technology. sciDROP NANO has a solenoid valve technology to generate droplets in the nL volume range. Print samples can be water-based solutions and organic solvents with surface tensions up to 28 mN/m

Versatile and Precise Nanoliter Dispensing for GENSPEED’s Multiplex Point of Care Testing System

To cover applications demanding large volume dispensing, SCIENION recently launched sciDROP NANO technology. sciDROP NANO has a solenoid valve technology to generate droplets in the nL volume range. Print samples can be water-based solutions and organic solvents with surface tensions up to 28 mN/m
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With sciPOLY3D proteins can be covalently attached to virtually all polymers without pre-treatment thereof and even on protein-repellent surfaces. sciPOLY3D is water soluble and is simply added to the printing media.

Protein microarrays on unmodified polymer supports using sciPOLY3D

With sciPOLY3D proteins can be covalently attached to virtually all polymers without pre-treatment thereof and even on protein-repellent surfaces. sciPOLY3D is water soluble and is simply added to the printing media.
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The applicability of sciPOLY3D for immobilization of DNA probes on a polymer surface is demonstrated in comparison to sciCHIP EPOXY glass slides. The epoxy chemistry is well established for the immobilization of aminefunctionalized DNA probes.

DNA microarrays on unmodified polymer supports using sciPOLY3D

The applicability of sciPOLY3D for immobilization of DNA probes on a polymer surface is demonstrated in comparison to sciCHIP EPOXY glass slides. The epoxy chemistry is well established for the immobilization of aminefunctionalized DNA probes.
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The development of an array-based multiplexed calves scours lateral flow assay has been chosen exemplarily as a case study for this application note.

Miniaturization and multiplexing of a lateral flow immunoassay for the detection of Rotavirus, Coronavirus, Escherichia coli F5 (K99) and Cryptosporidium in calf faeces

The development of an array-based multiplexed calves scours lateral flow assay has been chosen exemplarily as a case study for this application note.
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