cellenONE | Scienion

cellenONE

The open platform for single cell isolation and nanoliter dispensing

Real-time and high accuracy single cell isolation and dispensing

cellenONE® is a revolutionary technology based on optimized sciDROP PICO dispenser coupled with advanced visual processing solutions that provides real-time and high accuracy single cell isolation and dispensing.

Using gentle acoustic waves for droplet generation, cellenONE® preserves outstanding cell viability for all cloning applications and maintains protein expressions for sequencing applications.

Versatile technology for a variety of applications

Meanwhile, high resolution optics allows processing of a wide range of particles (including smaller than < 5µm), from bacteria to nuclei to microbeads to large mammalian cells, cellenONE® provides a versatile technology for a variety of applications.

cellenONE® provides also new frontiers in sample preparation prior to single cell analysis. Indeed, working in aspirate/dispense mode allows high recovery processing of a wide range of samples, from minute cell suspensions containing just a couple of microliters and a few dozen cells to much larger samples containing thousands of cells.

Benefits of cellenONE

  • High accuracy single cell isolation and dispensing

  • Gentle on cells, ensuring outstanding cell viability

  • Compatible with a wide range of particle sizes

  • Ideal for rare cell samples (zero dead volume and high recovery)

  • High-throughput isolation of hundreds of cells in only a few minutes

Benefits of cellenONE

  • High accuracy single cell isolation and dispensing

  • Outstanding cell viabilities

  • A wide range of particle sizes

  • Ideal for rare cell samples (zero dead volume and high recovery)

  • High throughput isolation of hundreds of cells in few minutes

Discover how cellenONE® technology isolates single cells in seconds

Applications

cellenONE® is a low volume, high viability and highly accurate single cell isolation technology for both sequencing and cell line development applications.

Single Cell Omics

Cell Line Development

We have worked successfully with a large variety of cell types:

Related application notes

Single Beads Dispensing Made Easy with PolyAn

Cell-line Development with TYTO sorting and cellenONE

Unparalleled clonal recovery achieved in 96 and 1536 MTP

For cloning applications with dendritics

Advanced software

cellenONE® X1 only dispenses single-cell containing drops into the microplate.

All remaining drops will be dispensed into a recovery tube, resulting in no sample wastage.

Ensure Single Cell Isolation

Visual Feedback and Software Mapping to Ensure Single Cell Isolation. A mapping report is automatically generated, it contains:

  • Evaluation of ejection zone boundary
  • Detected cell diameter, elongation and circularity
  • Average cell concentration
Droplet generation Acoustic wave
No. of Channels Up to 2
Dead volume Down to 0µL
Droplet volumes From 50 to 800pL
Dispenser materials Borosilicate glass
Cell Imaging HD camera for detection of cells and particles smaller than < 5µm
Isolation Speed One plate (96 single cells) in 4 min
Isolation Capacity Up to 2 MTPs (180×120 mm)
Dimensions 1300x700x1590 mm
Weight 205kg
  • Temperature, humidity and dew point control
  • Customized holders for microwell chips
  • Fiducial recognition and automated target alignment
  • Available consumables for calibration, sterilization, and recovery
Particle size From 2 to 70µm
Single cell accuracy Up to 100%
Single cell viability Up to 100%

cellenONE is a product developed by Cellenion, a subsidiary of SCIENION.

Visit the official Cellenion website for more information.