cellenONE
The open platform for single cell isolation and nanoliter dispensing
Real-time and high accuracy single cell isolation and dispensing
cellenONE® is a revolutionary technology based on optimized sciDROP PICO dispenser coupled with advanced visual processing solutions that provides real-time and high accuracy single cell isolation and dispensing.
Using gentle acoustic waves for droplet generation, cellenONE® preserves outstanding cell viability for all cloning applications and maintains protein expressions for sequencing applications.
Versatile technology for a variety of applications
Meanwhile, high resolution optics allows processing of a wide range of particles (including smaller than < 5µm), from bacteria to nuclei to microbeads to large mammalian cells, cellenONE® provides a versatile technology for a variety of applications.
cellenONE® provides also new frontiers in sample preparation prior to single cell analysis. Indeed, working in aspirate/dispense mode allows high recovery processing of a wide range of samples, from minute cell suspensions containing just a couple of microliters and a few dozen cells to much larger samples containing thousands of cells.
Benefits of cellenONE
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High accuracy single cell isolation and dispensing
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Gentle on cells, ensuring outstanding cell viability
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Compatible with a wide range of particle sizes
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Ideal for rare cell samples (zero dead volume and high recovery)
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High-throughput isolation of hundreds of cells in only a few minutes
Benefits of cellenONE
-
High accuracy single cell isolation and dispensing
-
Outstanding cell viabilities
-
A wide range of particle sizes
-
Ideal for rare cell samples (zero dead volume and high recovery)
-
High throughput isolation of hundreds of cells in few minutes
Discover how cellenONE® technology isolates single cells in seconds
Applications
cellenONE® is a low volume, high viability and highly accurate single cell isolation technology for both sequencing and cell line development applications.
Single Cell Omics
Cell Line Development
We have worked successfully with a large variety of cell types:
Related application notes
Single Beads Dispensing Made Easy with PolyAn
Cell-line Development with TYTO sorting and cellenONE
Unparalleled clonal recovery achieved in 96 and 1536 MTP
For cloning applications with dendritics
Advanced software
cellenONE® X1 only dispenses single-cell containing drops into the microplate.
All remaining drops will be dispensed into a recovery tube, resulting in no sample wastage.
Ensure Single Cell Isolation
Visual Feedback and Software Mapping to Ensure Single Cell Isolation. A mapping report is automatically generated, it contains:
- Evaluation of ejection zone boundary
- Detected cell diameter, elongation and circularity
- Average cell concentration
| Droplet generation | Acoustic wave |
| No. of Channels | Up to 2 |
| Dead volume | Down to 0µL |
| Droplet volumes | From 50 to 800pL |
| Dispenser materials | Borosilicate glass |
| Cell Imaging | HD camera for detection of cells and particles smaller than < 5µm |
| Isolation Speed | One plate (96 single cells) in 4 min |
| Isolation Capacity | Up to 2 MTPs (180×120 mm) |
| Dimensions | 1300x700x1590 mm |
| Weight | 205kg |
- Temperature, humidity and dew point control
- Customized holders for microwell chips
- Fiducial recognition and automated target alignment
- Available consumables for calibration, sterilization, and recovery
| Particle size | From 2 to 70µm |
| Single cell accuracy | Up to 100% |
| Single cell viability | Up to 100% |