Assay Development | Scienion

Assay Development

SCIENION offers innovative, complete and flexible solutions for multiplex assay development and optimization for any application in Diagnostics and Life Sciences.

Longstanding Experience

Customers can rely on our longstanding in-depth experience in all aspects of successful multiplex assay development, covering the whole range from surface functionalization, printing and immobilization, optimization of incubation protocols, detection and data analysis.

Developed Miniaturized Assays

Due to our unique sciFLEXARRAYER technology – the best choice for precise ultra-low volume liquid dispensing – customers benefit from developed cost-saving miniaturized assays. We also offer the transfer of already existing assays to the SCIENION platform – including multiplexing and miniaturization.

Assay Development Services

SCIENION’s Assay Development Services are offered on a modular work-packages basis to provide customers with a transparent and optimal solution for their needs. Depending on the customer’s requirements, we work with all typical supports like microplates, biosensors, wafers, slides, nitrocellulose membranes, lateral flow membranes and microfluidic chips that are made of glass, silicon dioxide, polymers or gold.

Assay development of DNA assays

  • Selection and screening of oligonucleotide probes
  • Isolation, purification and fluorescence labeling of target DNA
  • Development and optimization of hybridization protocols
  • DNA, oligonucleotides and PCR products
  • Proteins, peptides and antibodies
  • Glycans, glycolipids and glycopeptides
  • Cells, cell lysates
  • Nanoparticles, polymers and monomers

Assay development of protein assays

  • Development of incubation protocols for antibody/antigen detection
  • Development of miniaturized multiplex assays using planar array format in microplates
  • Development of washing, blocking and sealing protocols
  • Assay optimization for highest sensitivities, specificities and reproducibility

Assay miniaturization and multiplexing

  • Supply of materials and preparation of source plates
  • Microarray printing and processing (immobilization, blocking, washing)
  • Quality control of supports, array parameters and array functionality
  • Electronic documentation of the production process
  • Packaging according to customers requirements
  • OEM microarray production
  • Miniaturization of existing assays
  • Multiplexing of existing assays
  • Transfer of existing assays to SCIENION platform

Assay imaging and data analysis

  • Supply of materials and preparation of source plates
  • Microarray printing and processing (immobilization, blocking, washing)
  • Quality control of supports, array parameters and array functionality
  • Electronic documentation of the production process
  • Packaging according to customers requirements
  • OEM microarray production
  • Application of colorimetric and fluorescent detection methods
  • Raw data analysis including calculation of spot intensities and S/N ratios
  • Application of normalization routines
  • Quantitative analysis of arrays based on calibration methods, calculation of titration curves and assay parameters as LOD values
  • Customization of data analysis and reporting formats

 

Design of capture molecules, printing supports & biochips

Choice or optimization of capture molecules.

Choice of best support material according to the requested array format, detection methods and manufacturing costs.

Specifications of material parameters as surface roughness and planarity.

Surface functionalization

Functionalization of various materials such as glass, silicon, gold, polymers and membranes.

2D and 3D functionalized surfaces (e.g. carboxyl, aldehyde, amino, epoxy and hydrogel) for immobilization of biomolecules.

Tailor-made surface design by applying self assembling monolayer (SAM) and photografting technologies.

Printing & immobilization of capture probes

Functionalization of various materials such as glass, silicon, gold, polymers and membranes.

2D and 3D functionalized surfaces (e.g. carboxyl, aldehyde, amino, epoxy and hydrogel) for immobilization of biomolecules.

Tailor-made surface design by applying self assembling monolayer (SAM) and photografting technologies.

1. Define your capture probes

2. Pick your substrate

3. Specify your array layout

1. Define your capture probes

2. Pick your substrate

3. Specify your array layout

From Idea to Product

Full Test Development Service

SCIENION is your most competent partner for all development phases of bioanalytical tests – from designing a diagnostic assay up to its manufacturing. Upon demand, we solve the logistics to deliver your assays in-time around the globe.

1 - 4 weeks

6 - 24 month

6 - 12 month

3 month

After the Development Phase

After the completion of the development phase, we recommend our Contract Manufacturing Services for the high-throughput production of your diagnostic or other bioanalytical tests. Benefit from both: the scalability of our technologies and our ISO-certified manufacturing capabilities.