PCR in Nanoliter Volumes

PCR in nanoliter range is enabled by SCIENION technology through precision dispensing in the pico- and nanoliter range onton miniaturized microtiterplate format.

Small reaction volumes, typically being in the 2-200 nanoliter range, allow for ultrafast heating and cooling operations and to finish a complete PCR in as little as 10 minutes.

When compared to standard systems, typically only 10% of reagents are used in nanoliter PCR (nPCR). This leads especially in high throughput environments to massive cost reductions.

Combined with an online detection system for real-time or endpoint detection the combination described here allows for sensitive, cost-effective and quick analysis of nucleic acids.

Related application note

Increasing speed while decreasing cost: PCR in nanoliter volumes