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sciPOLY3D enables immobilization of biomolecules on most polymeric substrates, without the need for functional groups on the surface or the biomolecule.
sciPOLY3D enables covalent immobilization on protein-repellent surfaces, which reduces background and makes blocking obsolete.
sciPOLY3D is water-soluble and can thus be dispensed together with e.g. proteins or DNA probes.
It contains a photo reactive moiety, which upon UV irradiation couples the polymer chains to polymeric substrates, crosslinks the polymer chains leading to a polymer network and covalently attaches the biomolecules to the network.
Directly after the printing process, place the microarrays in a UV crosslinker (e.g. Stratalinker) with a wavelength of 254 nm, choose energy dose control with 1.25 J/cm2.
Due to the hydrophilic nature of the polymer, a surface-attached hydrogel with covalently embedded probe molecules is obtained.
The substrates do not need pretreatment, any native polymer substrate (e.g. PMMA, COP, COC, PP, PS, etc.) can be used.
To get started the Starter Kits are a very convenient option to try and test spotting of approx. 40 different samples and conditions. They are available for DNA and protein:
Preparation of a stock solution is recommended with 5 mg/ml sciPOLY3D in sciPOLY3D SOL1.
Producing a stock solution with 10 mg/ml is also an option, if needed. But be aware that in this condition a precipitate might form together with concentrated sciPOLY3D SOL2D1 buffer. The final concentration in the printing media generally is 1 mg/ml.
Last step in the synthesis procedure is the lyophilization of sciPOLY3D, therefore it comes as fluffy flakes, which easily dissolve in water. Vortexing for at least 2 times 2 minutes is recommended to yield a clear solution.
The stock solution can be stored for up to 4 weeks, if stored light protected and in a fridge.
|Product Name||Product Description||Contains||Product Number|
|sciPOLY3D SOLID||Polymer for 1-step printing Immobilization on non modified substrates. Requires ordering of sciPOLY3D SOL1||10 mg||CP-5802-10|
|sciPOLY3D SOL1||Solution buffer for sciPOLY3D SOLID||5 mL||CP-5804-5|
|sciPOLY3D LIQUID||Polymer for 1-step printing Immobilization on non modified substrates||0.5 mL 5x conc.||CP-5803-0.5|
|sciPOLY3D SOL2D1||Spotting buffer for sciPOLY3D with DNA||1 mL 2x conc.||CP-5805-1|
|100 mL 2x conc.||CP-5805-100|
|sciPOLY3D SOL2P1||Spotting buffer for sciPOLY3D with proteins||1 mL 2x conc.||CP-5806-1|
|100 mL 2x conc.||CP-5806-100|
|sciPOLY3D DNA||Starter kit for DNA||sciPOLY3D LIQUID (0.2 mL 5x conc.), sciPOLY3D SOL2D1 (0.5 mL 2x conc.) and 5x sciCHIP COP||CSP-5312-5|
|sciPOLY3D Protein||Starter Kit for proteins||sciPOLY3D LIQUID (0.2 mL 5x conc.), sciPOLY3D SOL2P1 (0.5 mL 2x conc.) and 5x sciCHOP COP||CP-5807|