Are you using the right buffer?

Discover our full range of buffers for DNA, oligos and proteins and improve the quality of your arrays

To take into account

Many diagnostic assays are designed at a pH of 7.4, since this is the normal pH of human blood.

However, for solid phase assays, like microarray applications, one has to distinguish between coating or spotting buffers and assay buffers.
There are different buffer requirements for these two process steps.

A spotting buffer has to guarantee:

  • maximum immobilization efficiency and uniform spot morphology while also,
  • maintaining the functionality of the capture biomolecule for the downstream assay requirements.

Also, it is not all about pH; if the immobilization chemistry is based on coupling of primary amines to functional groups on the surface, amine containing buffers like Tris must be avoided.
The assay buffer must guarantee optimal functionality of the involved biomolecules.

Usually, this is the case at physiological pH of 7.4.

The optimal buffer shows no interaction with the biomolecule of interest.

  • No phosphate based buffers should be used if the experiment involves an enzyme that is affected by phosphate, such as many kinases, phosphatases, dehydrogenases.
  • Borate based buffers should be avoided when working with glycoproteins or nucleotides, since Borate interacts with cis-hydroxyl moieties.
  • Did you know?

    The pH value varies with buffer concentration and with temperature.
    For example, the pH value of a 100 mM sodium phosphate buffer increases from 6.7 to 6.9 with 10-fold dilution.

    To guarantee a sufficient buffering capacity, a concentration of 25 – 50 mM is needed in most cases.
    If your assay employs enzymes which are sensitive to high ionic strength, you may try a lower concentration, e.g. 10 mM.



sciBUFFERs for DNA & Oligos

Product Name Product Description Contains Product Number
sciSPOT cDNA Printing buffer based on SSC (pH 7) with a detergent, to enable optimal microarray printing of cDNA samples. 50 mL 2x conc. CBD-5411-50
sciSPOT Oligo B1 Printing buffer based on SSC (pH 7) and is optimized for use with oligonucleotides on functionalized glass slides. 50 mL 2x conc. CBD-5421-50
sciSPOT Oligo B2 Printing buffer based on phosphate buffer (pH 9), for producing microarrays on polymer surfaces (sciPLEXPLATEs) 50 mL 2x conc. CBD-5418-50
sciPROCESS-cDNA Blocking buffers for post-printing treatment, used to deactivate the residual functional groups on the surface and minimize unspecific binding of the target DNA/RNA. 500 mL 2x conc. CBD-5416-500
sciPROCESS-Oligo   500 mL 2x conc. CBD-5426-500
sciPROCESS-Oligo   300 mL 2 x conc. CBD-5426-300
sciHYB Hybridization buffer to labeled cDNA or PCR products and immobilized capture probes, while minimizing cross-hybridization. Hybridization is accelerated and more efficient due to minimized secondary structures in probes and target sequences even at lower temperatures (42 °C). 1.5 mL 1x conc. CBD-5417-1.5
sciHYB M1 Hybridization buffer, that, in contrast to standard sciHYB, consists of a non-toxic formulation and is used at more elevated temperatures (55-65 °C). 50 mL 1 x conc. CBD-5419-50
sciWASH I DNA Washing buffers to reduce background signal after the hybridization step. Contain a detergent with ionic strengths for different levels of stringency. 500 mL 5x conc. CBD-5413-500
sciWASH II DNA   500 mL 5x conc. CBD-5414-500
sciWASH III DNA   500 mL 5x conc. CBD-5415-500
sciCOLOR T2 HRP substrate purpose-built for microarray applications 50 mL CD-5600-50
    100 mL CD-5600-100
    250 mL CD-5600-250
sciCOLOR T3 Substrate purpose-built for black staining microarray applications 50 mL CD-5603-50
    100 mL CD-5603-100
    250 mL CD-5603-250

sciBUFFERs SETs

Product Name Product Description Contains Product Number
sciBUFFERSET cDNA Set of buffers for optimal microarray printing of cDNA samples sciWASH I, II, III, sciPROCESS cDNA, sciHYB CBP-5410
sciBUFFERSET Oligo B1 Set of buffers for use with oligonucleotides on functionalized glass slides sciSPOT Oligo B1, sciWASH I, II, III, sciPROCESS Oligo CBP-5420
       
sciBUFFERSET Oligo M1 Set of buffers for use with oligonucleotides on polymer surfaces sciSPOT Oligo B2, sciPROCESS Oligo, sciHYB M1 CBP-5450

sciBUFFERs for Proteins

Product Name Product Description Contains Product Number
sciSPOT Protein D1 Printing buffer based on PBS at pH 7.2. PBS is a nontoxic buffer that mimics the osmolarity and ion concentrations of the human body. 25 mL 2x conc. CBP-5431-25
sciSPOT Protein D11 Printing buffer based on sciSPOT Protein D1, but contains an additive to slow down evaporation, which improves spot homogeneity. 25 mL 2x conc. CBP-5435-25
sciSPOT Protein D12 Printing buffer based on sciSPOT Protein D1, but includes an additive to stabilize proteins, which helps maintaining nativity of the proteins and promotes shelf life of the microarrays. 25 mL 2x conc. CBP-5436-25
sciSPOT Protein D2 Sodium Phosphate based buffer at pH 7.5. 25 mL 2x conc. CBP-5439-25
sciSPOT Protein D3 Printing buffer based on TBS, pH 8.0. 25 mL 2x conc. CBP-5441-25
sciSPOT Protein D4 Carbonate based printing buffer, pH 9.6 1.5 mL 1x conc. CBP-5442-25
sciSTAB S3 Protein stabilizer that can be added to any of our sciSPOT buffers. This will enhance shelf life and functionality of your protein microarrays by stabilizing the native structure of the arrayed proteins. 2 mL 5 x conc. CD-5601-2
sciBLOCK Protein D1 Designed for post-print processing of the protein microarrays and will passivate the surface. This PBS based buffer efficiently blocks unspecific binding, resulting in low background. 500 mL 5x conc. CBP-5433-500
sciBLOCK Protein D1M   100 mL 1x conc. CBP-5438-100
sciWASH Protein D1 PBS based washing buffer, suitable for all stages during multiplex ELISA. 500 mL 8x conc. CBP-5432-500
sciWASH Protein D1M   250 mL 5x conc. CBP-5437-250
sciBIND Protein D1 PBS based reagent diluent, which is ideally suited for dilution of your samples and secondary antibodies. This buffer is formulated to enhance your signals while reducing cross-reactivity. 1.6 mL 2x conc. CBP-5434-1.6
    50 mL 2x conc. CBP-5434-50
sciCOLOR T2 HRP substrate purpose-built for microarray applications 50 mL CD-5600-50
    100 mL CD-5600-100
    250 mL CD-5600-250
sciCOLOR T3 Substrate purpose-built for black staining microarray applications 50 mL CD-5603-50
    100 mL CD-5603-100
    250 mL CD-5603-250
sciSTAB S3 Protein stabilizer that can be added to any of our sciSPOT buffers. This will enhance shelf life and functionality of your protein microarrays by stabilizing the native structure of the arrayed proteins. 2 mL 5x conc. CD-5601-2

sciBUFFERs SETs

Product Name Product Description Contains Product Number
sciBUFFERSET Protein D1 Set of buffers for optimal microarray printing of protein samples sciSPOT Protein D1, sciWASH Protein D1, sciBLOCK Protein D1, sciBIND Protein D1 CBP-5410
sciBUFFERSET Protein D1M Set of buffers for optimal microarray printing of protein samples, up to 5 x96 well plates sciSPOT Protein D1, sciWASH Protein D1M, sciBLOCK Protein D1M, sciBIND Protein D1M CBP-5440
sciMULTIPLEX Box sciMULTIPLEX system contains selected consumables to facilitate transfer of multiple traditional singleplex immunoassays to one multiplex immunoassay. The intention of this kit is to expedite setting up a proof-of-principle assay as a solid base for further development and validation. 2x sciSOURCEPLATE 384 PP, 2 sciPLEXPLATE Type 1, 2x Type 2 and 2x solid black, sciSPOT Protein D1, D2, D3, D4 (5 mL each at 2 conc.), sciSTAB S3, sciBLOCK Protein D1M, 2x sciWASH Protein D1 (75 mL, 8x conc.) and sciCOLOR T2 CD-5602