Protein & Peptide Biochips

Multiplex ELISA

Conventional (left) vs. 5 x 5 multiplex

High quality multiplex immunoassays can be easily achieved in 96 or 384-well plates where each spot results in a different assay.

Non-specific antigen or antibody binding can be minimized with proper optimization.

The very high assay capacity enables replicates for excellent statistical results analysis.

The large dynamic range (4 logs) with pg/ml sensitivity leads to reduced sample and reagent consumption, lower assay costs.

High Throughput Protein-Protein Interaction Studies

Courtesy of Andrea Fiebitz, MPI Molecular Genetics

DNA samples are spotted and immobilized on glass slides in array formats. Each DNA spot contains bait and prey expression plasmids in addition to a reporter plasmid coding for an auto-fluorescent protein.

After spotting the vector constructs adherent human cells are added, creating a monolayer on the surface of the slides.

Only cells growing on top of the DNA spots become transfected. In the case of chimeric bait and prey protein interaction the reporter gene is expressed, resulting in formation of fluorescent reporter protein.

Signals resulting from PPI can be analyzed directly by fluorescent detection.