PCR in Nanoliter Volumes

The combination of precise dispensing in the pico- and nanoliter range and miniaturized microtiterplate format enables to perform PCR in nanoliter volumes.

Small reaction volumes, typically being in the 2-200 nanoliter range, allow for ultrafast heating and cooling operations and to finish a complete PCR in as little as 10 minutes.

When compared to standard systems, typically only 10% of reagents are used in nanoliter PCR (nPCR). This leads especially in high throughput environments to massive cost reductions.

Combined with an online detection system for real-time or endpoint detection the combination described here allows for sensitive, cost-effective and quick analysis of nucleic acids.