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The combination of precise dispensing in the pico- and nanoliter range and miniaturized microtiterplate format enables to perform PCR in nanoliter volumes.
Small reaction volumes, typically being in the 2-200 nanoliter range, allow for ultrafast heating and cooling operations and to finish a complete PCR in as little as 10 minutes.
When compared to standard systems, typically only 10% of reagents are used in nanoliter PCR (nPCR). This leads especially in high throughput environments to massive cost reductions.
Combined with an online detection system for real-time or endpoint detection the combination described here allows for sensitive, cost-effective and quick analysis of nucleic acids.