Cell Based Assays

3D Cell Cultures

Hydrogels are becoming more and more popular as platforms for three-dimensional (3D) cell culture. 3D hydrogel matrices have been used for a variety of applications, including tissue engineering of micro-organ systems, drug delivery, cytotoxicity testing, and drug screening. Moreover, 3D cell culture is applied for investigating cellular physiology, stem cell differentiation, tumor models and for studying interaction mechanisms between cells and extracellular matrix.

HeLa cells encapsulated in alginate beads suspended in DMEM solution and visualized through a glass coverslip. The alginate beads were generated using sciDROP PICO technology mounted on a sciFLEXARRAYER S3 (SCIENION AG, Germany).

 

 

Co-culture strategies are foundational in cell biology. These systems, which serve as mimics of in vivo tissue niches, are typically poorly defined in terms of cell ratios, local cues and supportive cell–cell interactions. In the stem cell niche, the ability to screen cell–cell interactions and identify local supportive microenvironments has a broad range of applications in transplantation, tissue engineering and wound healing. We present a microfluidic platform for the high-throughput generation of hydrogel microbeads for cell co-culture. Encapsulation of different cell populations in microgels was achieved by introducing in a microfluidic device two streams of distinct cell suspensions, emulsifying the mixed suspension, and gelling the precursor droplets. High-throughput combinatorial cell co-culture using microfluidics, 28 Apr 2011, Ethan Tumarkin et al.

Drug Screening

After spotting the cells on e.g. 500 µm spots using the sciFLEXARRAYER cells can be cultivated directly on the target.

The specific treatment can be performed with the drop-in-drop printing.

With up to 100 cells per drop, cell behavior at the individual cell level can be analysed, using high resolution fluorescence microscopy and automated image analysis.

Cell arrays could enable high throughput and large scale in-vitro drug testing.

Multiplexed Toxicity Assays

The development of high-throughput and highly relevant in vitro toxicity assays can help limiting the extend of animal testing.

An in-vitro method for toxicity testing can be the combining of bioassay and morphometric endpoints.